SENNA MACRANTHERA AS AN ALTERNATIVE TO CONTROL BOVINE MASTITE

SENNA MACRANTHERA AS AN ALTERNATIVE TO CONTROL BOVINE MASTITE

Flavia Inouea, Raoni Pais Siqueiraa, Gaspar Diazb, Virginia Ramos Pizzioloa, Marisa Alves Nogueira Diaza*aDepartamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs s/n 36570-000, Viçosa-MG, Brazil. marisanogueira@ufv.brbInstituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, 31270-901, Belo Horizonte-MG, Brazil

INTRODUCTION

Bovine mastitis, an inflammatory response in cow’s udder, is the main infecto-contagious disease affecting dairy cattle and is considered a limiting factor in many dairy properties. The huge economic losses associated with mastitis worldwide motivate researches focused on several aspects of the disease. Staphylococcus aureus is the main etiologic causative agents of mastitis and Infections caused by this microorganism frequently turn into chronic and cows with such infections have to be culled (1). Senna macranthera (Fabaceae) is a native plant from Brazil frequently found in the semi-deciduous highland forest with occurrence from Ceará to São Paulo and Minas Gerais. It is used for the regeneration of degraded areas and as ornamental tree. This plant is popularly known as, “pau fava”, “cássia”, “cássia-macrantera”, “fedegoso” and “macrantera”. In Brazil it has been used as a drug for infectious diseases.This study aims to evaluate the antimicrobial activity of polar and non-polar extracts from roots and flowers of S. macranthera and also to evaluate sanitation potentials of this plant based herbal soap formulated in our laboratory as personal care products (PCP).

MATERIAL AND METHOD

Plant material Roots and Flowers from S. macranthera were collected in campus of Federal University of Viçosa in 2010. The roots and leaves were dried at 40 °C for 24 h. Then, 400 g of roots and XX of Flowers were extracted using dichloromethane, Ethyl acetate and ethanol respectively . The extract were concentrated to dryness under reduced pressure and stored at 4 °C.Antibacterial screening assayHole-plate diffusion assay was initially performed to test for antibacterial activity of the six extracts. For such purpose, the bacteria were cultivated overnight and suspension containing 106 CFU mL-1 was spread in plates containing Müeller-Hinton agar (Himedia®). Holes of approximately 5 x 3 mm were made in the agar and filled with 30 μL of the stock solutions (50 mg mL-1). After incubation at 37 °C for 24 h, inhibition zones were measured in millimeters and compared to the controls. The antibiotic ciclopyrox olamine (Uci-Farma®) was used as the positive control due to its antibacterial properties. Dimethylsulfoxide (DMSO) was used as negative control. Tests were performed twice in triplicate. The minimum inhibitory concentration concentration (MIC) of the positive extracts were determined by a broth microdilution method followed by incubation at 37°C for 24 h and observation of media turbidity.

Formulation of Herbal SoapThe dichloromethane extract from roots of S. macranthera (250 mg) was incorporated into a soap formulated according patent PI 1005633-5 (2). After, the semi-solid mixture was poured into a mold and allowed to solidify. A soap without extract was produced and used

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